Determination of the low-abundant protein biomarker hCG from dried matrix spots using immunocapture and nano liquid chromatography mass spectrometry
نویسندگان
چکیده
منابع مشابه
Performing Quantitative Determination of Low- Abundant Proteins by Targeted Mass Spectrometry Liquid Chromatography
Mass spectrometry coupled to nanoliquid chromatography aims to be an alternative to antibody‐based determination of low‐abundant proteins. High‐resolution mass spec‐ trometers, plug‐and‐play systems and pumps have been developed for this purpose. Important aspects of approaches are limit of detection, specificity, variability and cost. In this chapter, the most recent literature (from 2008) has...
متن کاملTryptic peptide analysis of ceruloplasmin in dried blood spots using liquid chromatography-tandem mass spectrometry: application to newborn screening.
BACKGROUND Newborn screening to identify infants with treatable congenital disorders is carried out worldwide. Recent tandem mass spectrometry (MS/MS) applications have markedly expanded the ability to screen for >50 metabolic diseases with a single dried blood spot (DBS). The feature that makes metabolic disorders particularly amenable to screening is the presence of specific small-molecule me...
متن کاملMultiplex Assay of Second-Line Anti-Tuberculosis Drugs in Dried Blood Spots Using Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry
As dried blood spots (DBSs) have various advantages over conventional venous blood sampling, some assays for detection of one or two anti-tuberculosis (TB) drugs in DBSs have been developed. However, there are no assays currently available for the simultaneous measurement of three or more anti-TB drugs in DBSs. In this study, we developed and evaluated a multiplex method for detecting nine anti...
متن کاملDetermination of Mebudipine in Human Plasma by Liquid Chromatography–tandem Mass Spectrometry
In previous studies, mebudipine, a dihydropyridine calcium channel blocker, showed a considerable potential to be used in cardiovascular diseases. The aim of the current study was to develop a valid method using reversed-phase high performance liquid chromatography coupled with electrospray ionization mass spectrometry to assay mebudipine in the human plasma. Separation was achieved on a Zorbax...
متن کاملDevelopment and Application of a Validated Liquid Chromatography-Mass Spectrometry Method for the Determination of Dexchlorpheniramine Maleate in Human Plasma
A convenient liquid chromatographic-single Quadrupole mass spectrometric (LC-MS) method was developed and validated for dexchlorpheniramine maleate (INN name: chlorphenamine) determination in human plasma. The need for just a single liquid-liquid extraction with ethyl acetate and being highly sensitive were the advantages of this method. The linearity was also excellent over the range of 1...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Chromatography B
سال: 2018
ISSN: 1570-0232
DOI: 10.1016/j.jchromb.2018.01.016